Agilent AdvanceBio Sialidase V, recombinant 1 U; GK80060

Agilent Specifications:- Concentration: 5 U/mL. Enzyme Applications: Glycan analysis, glycan digestion, sialic acid analysis, sialic acid removal, desialylation. Enzyme Formulation: Sterile-filtered solution in 50 mM sodium acetate, 150 mM NaCl, pH 5.5. Enzyme Source: Recombinant, expressed in E. coli, from Vibrio cholerae. Enzyme Specificity: Cleaves all nonreducing terminal Alpha (2-3,6,8)-linked-sialic acid from oligosaccharides, glycoproteins, and complex carbohydrates. Enzyme Unit Definition: One unit of AdvanceBio Sialidase V is defined as the amount of enzyme required to catalyze the release of 1 umol of methylumbelliferone from MU-NANA [2'-(4-methylumbelliferyl)- Alpha -d-N-acetylneuraminic acid] per minute at pH 5.5 and 37 C. Storage Temp Conditions: Refrigeration required, 2-8 C. Volume: 200 uL. - BC