Agilent Pfu Dna Ligase, 400u; 600191

Agilent Technologies Pfu Dna Ligase, 400u; Kit Contains: 400 U Pfu Dna Ligase 10x Reaction Buffer - Pfu Dna Ligase
Pfu Dna Ligase Is A Recombinant Enzyme Derived From Pyrococcus Furiosus Hyperthermophilic Marine Archaebacterium That Catalyzes Linkage Of Adjacent 5-Phosphate And 3-Hydroxy Ends Of Double-Stranded Dna At 45 To 80 °C, With A Temperature Optimum Near 70 °C For Nick-Sealing Reactions.
Highly Thermostable, With Half-Life Of Over 60 Minutes At 95 °Cincreases Reliability Of Lcr By Permitting Higher Melt Temperatureseliminates Lcr Template-Predenaturation Step, Less Enzyme Required For Lcr
It Has Higher Ligation Specificity And Lower Background Than Tth Dna Ligase.
- SR (Additional S&H Or Hazmat Fees May Apply)